How TrueAllele® Works, Part 2: Degraded DNA and allele dropout

• 20-Nov-2014, 1pm EDT
  
  

  TrueAllele Casework separates DNA mixtures into genotypes, solves kinship and paternity, and calculates match statistics. This half hour talk will focus on how TrueAllele handles degraded DNA and allele dropout.

  Speaker: Dr. Mark Perlin

  
  

PowerPoint presentation

PowerPoint handout

Question and Answer Session





Note

Unlike TrueAllele, some STR mixture analysis methods discard peak data below a certain "threshold" height. The study by Kloosterman set a 10 rfu threshold (baseline noise) for TrueAllele, and a 50 rfu threshold for the other methods. Using a threshold, or adjusting its level, can significantly change the match statistic, as mentioned in the talk.