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TrueAllele® Casework takes NGS from the laboratory into the courtroom

William P. Allan, MS, Kari R. Danser, MS, Mark W. Perlin, PhD, MD, PhD, "TrueAllele® Casework takes NGS from the laboratory into the courtroom", Mid-Atlantic Association of Forensic Scientists Annual Meeting, Richmond, VA, 9-May-2025.


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Abstract

Next generation sequencing (NGS) is a modern modality for laboratory DNA analysis. Hundreds, or hundreds of thousands, of genetic loci can be simultaneously examined on a single evidence item. TrueAllele® probabilistic genotyping technology has been validated on NGS for complex DNA mixtures. But how can a crime lab use NGS data with PG software in court? This talk walks through the steps, showing how to interpret NGS data, produce match statistics, prepare a case report, and deliver courtroom testimony.


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